If you click on Deny All when you do Forum Permissions, then any new usergroups you create should be set to Deny for that forum. If you manually went through and denied every usergroup to that forum, then you are correct in that when you create a new usergroup, it will be allowed. (The weird thing is, which I wish was not this way, the new usergroup doesn't show up in RED in the forum list, but the little circle next to the group will be an open circle, not a closed dot, and the open circle means they can't see the forum.)
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