Is your image in layers? I dont know to much about saving in photoshop but I know in psp you have to save as png to keep the transparency. IF you need .gif you have to open it in gimp and save your psp PNG to .gif that way. Or it will just merge to white background.
If its not in layers you need to use the background eraser tool. again.. I use PSP.
Post the image, maybe I can help!
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